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Define flow cytometry8/2/2023 The Bethesda Consensus does provide recommended markers for each category of case types. The lab's fresh tissue panel is identical to the above, but omits tubes 6, 7, and 8. There is currently no standard recommended set of marker combinations. The antibodies and combinations were selected based on the preference of one particular laboratory. Identify T cell and plasma cell malignancyĪbove is an example panel used for peripheral blood and bone marrow samples. Idenify and characterize B cell malignancy A common anchor antibody, typically CD45, is present throughout all of the tubes. The antibodies are generally grouped in lineage specific groupings. For instance, in the diagram below, you can see that CD19 is the earliest B specific protein expressed on B cells, but that it is lost as the activated B cell becomes a plasma cell. Most of these antibodies are against surface proteins that are not only often associated with particular cell lineages, but vary in expression with maturation, and thus are referred to as differentiation antigens. This information is invaluable for selecting therapy and for determining prognosis and can help in the detection of relapse or of secondary leukemia. ![]() The use of immunologic markers to determine the lineage and degree of differentiation of a leukemia has become an essential part of the diagnostic evaluation. Combinations of antibodies allow for 1) identification of specific cell types, 2) determination of the degree of cell differentiation, and 3) recognition of abnormal cells. how many different wavelengths the instrument can acquire simultaneously) this will usually be divided to between 3-10 tubes per sample. ![]() Depending on the capabilties of the instrument (i.e. This page will just provide a general overview of the principles and thought process behind an analysis.Ī typical sample is usually stained with between 10-30 monoclonal antibodies or CD markers. These cases contain a detailed explanation about each tube and what are the key features to look for on each tube. We have several normal cases posted that you can download and view using the FCS Express Reader. ![]() The following is an introduction to the principles of flow cytometric analysis of hematolymphoid neoplasia.
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